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Arya, I. D.
- Micropropagation of Two Economically Important Bamboos : Drepanostachyum falcatum (NEES) Keng and Bambusa balcooa Roxb.
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Explant, Shoot Multiplication, In-vitro Rooting
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Indian Forester, Vol 134, No 9 (2008), Pagination: 1211-1221Abstract
A protocol for the micropropagation of two economically important bamboos, Drepanostachyum falcatum and Bambusa balcooa, which have some problems in their conventional propagation, was developed. Nodal explants with single axillary buds were excised from mother plant and were washed with cetavelon. Initially high incidence of fungal contamination was observed in most cultures of Bambusa balcooa, which was controlled by using Bavistin (1%) for 5-7 minutes followed by surface sterilization for 8 -12 minutes with 0.1% HgCl2. Liquid MS medium supplemented with 5.0 mg/l 6-Benzyl Amino Purine (BAP) for Drepanostachyum falcatum and 3.0-5.0 mg/l BAP for Bambusa balcooa was found to be the best for axillary bud induction. The multiple shoots were excised from mother explant and further multiplied on MS medium supplemented with defined plant growth regulators. Best shoot multiplication was observed on MS medium supplemented with 3.0 mg/l BAP for Drepanostachyum falcatum and 3.0 mg/l BAP with 0.5 mg/l Kinetin for Bambusa balcooa. The morphogenetic potential of the axillary buds was adversely affected by phenolic exudates especially in Bambusa balcooa. This was overcome by transferring the explant to the fresh medium after every 12-15 days or whenever the medium turned brown. While a regular subculture in every 3-4 weeks increased the multiplication rate in Drepanostachyum falcatum. In-vitro shoots were ischolar_mained when transferred to MS medium supplemented with auxin (IBA, NAA and IAA). The ischolar_mained plantlets of Drepanostachyum falcatum were hardened, acclimatized and successfully transferred to field. However the experiments pertaining to optimal ischolar_maining response and hardening of Bambusa balcooa are in progress.Keywords
In-vitro, Axillary Bud, Drepanostachyum falcatum, Bambusa balcooa, NodalExplant, Shoot Multiplication, In-vitro Rooting
- Flowering in Exotic Bamboo Dendrocalamus asper in India
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Indian Forester, Vol 127, No 9 (2001), Pagination: 1053-1057Abstract
No abstract- Tissue Culture Technology for Rapid Multiplication of Dendrocalamus giganteus Munro
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Indian Forester, Vol 132, No 3 (2006), Pagination: 345-357Abstract
Micropropagation of Dendrocalamus giganteus through axillary bud culture is described. Multiple shoots were induced from in-vitro culture of nodal shoot segments containing axillary bud. Nodal segments produced axillary shoots in 2-3 weeks on Murashige and Skoog's (MS) medium supplemented with BAP (10-40 μ M). These shoots were excised from mother explants and subcultured on different concentrations of BAP in MS medium. Highest shoot multiplication rate of 5.44 fold was obtained on 20 μ M BAP medium. On MS medium supplemented with Kn only 3-4 fold shoot multiplication was obtained. The addition of BAP (10 μ M) in the kinetin (10 μ M) supplemented medium enhanced the shoot multiplication rate to 6.35 fold. 16-66 % ischolar_mains were formed on excised propagules of 3-5 shoots when transferred to MS medium supplemented with 15-25 μ M IBA. The maximum ischolar_mains per propagule (8.66) and ischolar_maining percentage (90 %) was obtained on MS medium supplemented with 25 μ M IBA + 0.05 μ M BAP. These tissue culture raised plants were successfully hardened and acclimatized and transferred to field condition.- Effect of Sowing Depth and Media on Seed Germination of Ailanthus excelsa Roxb
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Authors
Affiliations
1 Department of Agriculture and Co-operation, Regional Pesticides Testing Laboratory, Chandigarh, IN
2 Forest Genetics and Tree Breeding Division, Arid Forest Research Institute, Jodhpur, Rajasthan, 342001, IN
3 Forest Genetics and Tree Breeding Division, Arid Forest Research Institute, Jodhpur, Rajasthan-342001, IN
1 Department of Agriculture and Co-operation, Regional Pesticides Testing Laboratory, Chandigarh, IN
2 Forest Genetics and Tree Breeding Division, Arid Forest Research Institute, Jodhpur, Rajasthan, 342001, IN
3 Forest Genetics and Tree Breeding Division, Arid Forest Research Institute, Jodhpur, Rajasthan-342001, IN